Technical Advance Efficient and ultrasensitive identification of clinical somatic mutations by modified single duplex DNA sequencing

Conventional next-generation sequencing (NGS) implementations have poor sensitivity for low prevalence mutations due to their inherent error rate. Error suppression that reconciles individual strands of a DNA duplex can improve sensitivity. Concatenating Original Duplex for Error Correction (CODEC) is an emerging technology that physically links duplex strands to enable their comparison from a single read pair, circumventing high sequencing depths normally required to recover strands independently.